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Image Search Results
Journal: Nucleic Acids Research
Article Title: CsrA maximizes expression of the AcrAB multidrug resistance transporter
doi: 10.1093/nar/gkx929
Figure Lengend Snippet: Effect of CsrA on acrA -GFP translation. Coupled transcription-translation reactions were performed with a PURExpress kit using pT7- acrA -GFP and pT7- acrA -GFP Mut CsrA BS translational fusions in the presence and absence of purified CsrA-His protein (320 nM). Fluorescence was measured at excitation and emission wavelengths of 492 and 520 nm, respectively using a FLUOstar Optima. Each experiment was performed three times.
Article Snippet: These two plasmids were used as the templates for coupled transcription-translation reactions using the
Techniques: Purification, Fluorescence